Kinetic Properties Of Wheat Germ Aldolase

Kinetic Properties of Wheat Germ Phosphatase Results: 1. The final concentration of pane of glass Phosphatase enzyme in this reaction was 0.07 ìM, the final inhibitor concentration was 0.67 ìM. (see sample computing unmatchable). 2. tabular array 1. Enzyme-Substrate reception (Un contain) render         Concentration of PNPP (mM)          garishness of 5.0mM PNPP (ml)         Volume of dH2O (ml)         A405          hurrying*(ì jetty/min) 1         0         0         4.3         0 (blank)         0 2         0.05         0.05         4.25         0.148         0.0118 3         0.075         0.075         4.225         0.199         0.0159 4         0.113         0.113        Â Â 4.187         0.231         0.0184 5         0.170         0.170         4.130         0.286         0.0228 6         0.255         0.255         4.045         0.337         0.0269 7         0.384         0.384         3.916         0.398         0.0318 8         0.578         0.578         3.722         0.414         0.0330 9         0.88         0.88         3.42         0.507         0.0405 10         1.32         1.32         2.98         0.578         0.0461 11         2.0         2.0         2.3         0.655         0.0523 12      Â!  Â Â 3.0         3.0         1.3         0.708         0.0565 7         0.384         0.384         3.916         0.324         0.0259 * See Sample Calculation #2. 7 is a send of tube #7. 3. control panel 2. Enzyme-Substrate Reaction (Inhibited) Tube         Concentration of PNPP (mM)         Volume of 5.0mM PNPP (ml)         Volume of 1.5mM K2HPO4 (ml)         Volume of dH20 (ml)         A405         Velocity(ìmol/min) 1         0         0         1         3.3         0 (blank)         0 2         0.05         0.05         1         3.25         0.002         0.0002 3         0.075         0.075   Â Â Â Â Â Â Â 1         3.225         0.002         0.0002 4         0.11         0.11         1         3.19         0.023         0.0018 5         0.17         0.17         1         3.13         0.070         0.0056 6         0.26         0.26         1         3.04         0.081         0.0065 7         0.38         0.38         1         2.91         0.103         0.

0082 8         0.58         0.58         1         2.72         0.116         0.0093 9         0.88         0.88         1         2.42         0.161         0.0128 10         1.32         1.32         1         1.98         0.226         0.0180 11         2.00         2.00         1         1.3         0.302         0.0241 12         3.00         3.00         1         0.3         0.418         0.0334 3. Graph one 4. Graph two 5. Table 3: Km and Vmax set Plot type         Km uninhibited         Km inhibited          Vmax uninhibited         Vmax inhibited MM*         0.28         4.00         0.72         0.95 LB         0.15         31.09         0.047         0.62 * See abandoned Kinetic printouts. Sample Calculations 1.         Enzyme: V1M1 = V2M2 (0.2ml)(2.5ìM Acid Phosphatase) = (7.5ml)(M2) M2 = 0.07 ìM Inhibitor: V1M1 = V2M2 (1ml)(5mM K2HPO4) = (7.5ml)(M2) M2 = 0.67 ìM 2. Velocity (aka. Enzyme Activity) Example: Tube 2 of Uninhibited a.          hold absorbance to find concentration. c = A/(ål) = (0.148)/[(18.8x103L/mol-cm)(1cm)] = 7.87 x... If you want to get a full essay, order it on our website: BestEssayCheap.com

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